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kpa substrates  (Addgene inc)


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    Addgene inc kpa substrates
    Impact of ECM stiffness on mitochondrial calcium influx. (A, C) Live imaging time-lapse series capturing the intensity of calcium-specific biosensors within HeLa cells: pCMV CEPIA2 mt for mitochondrial calcium (green) and pCMV R-CEPIA1er for endoplasmic reticulum (ER) calcium (red), post-treatment with 1 µM ionomycin on <t>substrates</t> with 1 and 40 kPa stiffness. Ionomycin was administered 5 min after initiating the imaging process, with only the media and cells present in the confocal dish during the initial 5 min. The accompanying color scale bar represents calcium levels, with red indicating high and blue denoting low calcium concentrations (scale bar = 10 µm). (B, D) Quantitative analysis of the time courses of normalized emission intensity from the pCMV CEPIA2 mt and pCMV R-CEPIA1er biosensors in HeLa cells treated with 1 µM ionomycin on substrates with 1 and 40 kPa stiffness. Each line represents the mean value of normalized emission intensity for a group of 10 cells (n = 10), and the error bars reflect the standard error of the mean (SEM), illustrating the dynamic calcium changes in response to ECM stiffness and ionomycin treatment.
    Kpa Substrates, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kpa substrates/product/Addgene inc
    Average 93 stars, based on 3 article reviews
    kpa substrates - by Bioz Stars, 2026-04
    93/100 stars

    Images

    1) Product Images from "ECM stiffness regulates calcium influx into mitochondria via tubulin and VDAC1 activity"

    Article Title: ECM stiffness regulates calcium influx into mitochondria via tubulin and VDAC1 activity

    Journal: Animal Cells and Systems

    doi: 10.1080/19768354.2024.2393811

    Impact of ECM stiffness on mitochondrial calcium influx. (A, C) Live imaging time-lapse series capturing the intensity of calcium-specific biosensors within HeLa cells: pCMV CEPIA2 mt for mitochondrial calcium (green) and pCMV R-CEPIA1er for endoplasmic reticulum (ER) calcium (red), post-treatment with 1 µM ionomycin on substrates with 1 and 40 kPa stiffness. Ionomycin was administered 5 min after initiating the imaging process, with only the media and cells present in the confocal dish during the initial 5 min. The accompanying color scale bar represents calcium levels, with red indicating high and blue denoting low calcium concentrations (scale bar = 10 µm). (B, D) Quantitative analysis of the time courses of normalized emission intensity from the pCMV CEPIA2 mt and pCMV R-CEPIA1er biosensors in HeLa cells treated with 1 µM ionomycin on substrates with 1 and 40 kPa stiffness. Each line represents the mean value of normalized emission intensity for a group of 10 cells (n = 10), and the error bars reflect the standard error of the mean (SEM), illustrating the dynamic calcium changes in response to ECM stiffness and ionomycin treatment.
    Figure Legend Snippet: Impact of ECM stiffness on mitochondrial calcium influx. (A, C) Live imaging time-lapse series capturing the intensity of calcium-specific biosensors within HeLa cells: pCMV CEPIA2 mt for mitochondrial calcium (green) and pCMV R-CEPIA1er for endoplasmic reticulum (ER) calcium (red), post-treatment with 1 µM ionomycin on substrates with 1 and 40 kPa stiffness. Ionomycin was administered 5 min after initiating the imaging process, with only the media and cells present in the confocal dish during the initial 5 min. The accompanying color scale bar represents calcium levels, with red indicating high and blue denoting low calcium concentrations (scale bar = 10 µm). (B, D) Quantitative analysis of the time courses of normalized emission intensity from the pCMV CEPIA2 mt and pCMV R-CEPIA1er biosensors in HeLa cells treated with 1 µM ionomycin on substrates with 1 and 40 kPa stiffness. Each line represents the mean value of normalized emission intensity for a group of 10 cells (n = 10), and the error bars reflect the standard error of the mean (SEM), illustrating the dynamic calcium changes in response to ECM stiffness and ionomycin treatment.

    Techniques Used: Imaging



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    Impact of ECM stiffness on mitochondrial calcium influx. (A, C) Live imaging time-lapse series capturing the intensity of calcium-specific biosensors within HeLa cells: pCMV CEPIA2 mt for mitochondrial calcium (green) and pCMV R-CEPIA1er for endoplasmic reticulum (ER) calcium (red), post-treatment with 1 µM ionomycin on <t>substrates</t> with 1 and 40 kPa stiffness. Ionomycin was administered 5 min after initiating the imaging process, with only the media and cells present in the confocal dish during the initial 5 min. The accompanying color scale bar represents calcium levels, with red indicating high and blue denoting low calcium concentrations (scale bar = 10 µm). (B, D) Quantitative analysis of the time courses of normalized emission intensity from the pCMV CEPIA2 mt and pCMV R-CEPIA1er biosensors in HeLa cells treated with 1 µM ionomycin on substrates with 1 and 40 kPa stiffness. Each line represents the mean value of normalized emission intensity for a group of 10 cells (n = 10), and the error bars reflect the standard error of the mean (SEM), illustrating the dynamic calcium changes in response to ECM stiffness and ionomycin treatment.
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    Image Search Results


    Impact of ECM stiffness on mitochondrial calcium influx. (A, C) Live imaging time-lapse series capturing the intensity of calcium-specific biosensors within HeLa cells: pCMV CEPIA2 mt for mitochondrial calcium (green) and pCMV R-CEPIA1er for endoplasmic reticulum (ER) calcium (red), post-treatment with 1 µM ionomycin on substrates with 1 and 40 kPa stiffness. Ionomycin was administered 5 min after initiating the imaging process, with only the media and cells present in the confocal dish during the initial 5 min. The accompanying color scale bar represents calcium levels, with red indicating high and blue denoting low calcium concentrations (scale bar = 10 µm). (B, D) Quantitative analysis of the time courses of normalized emission intensity from the pCMV CEPIA2 mt and pCMV R-CEPIA1er biosensors in HeLa cells treated with 1 µM ionomycin on substrates with 1 and 40 kPa stiffness. Each line represents the mean value of normalized emission intensity for a group of 10 cells (n = 10), and the error bars reflect the standard error of the mean (SEM), illustrating the dynamic calcium changes in response to ECM stiffness and ionomycin treatment.

    Journal: Animal Cells and Systems

    Article Title: ECM stiffness regulates calcium influx into mitochondria via tubulin and VDAC1 activity

    doi: 10.1080/19768354.2024.2393811

    Figure Lengend Snippet: Impact of ECM stiffness on mitochondrial calcium influx. (A, C) Live imaging time-lapse series capturing the intensity of calcium-specific biosensors within HeLa cells: pCMV CEPIA2 mt for mitochondrial calcium (green) and pCMV R-CEPIA1er for endoplasmic reticulum (ER) calcium (red), post-treatment with 1 µM ionomycin on substrates with 1 and 40 kPa stiffness. Ionomycin was administered 5 min after initiating the imaging process, with only the media and cells present in the confocal dish during the initial 5 min. The accompanying color scale bar represents calcium levels, with red indicating high and blue denoting low calcium concentrations (scale bar = 10 µm). (B, D) Quantitative analysis of the time courses of normalized emission intensity from the pCMV CEPIA2 mt and pCMV R-CEPIA1er biosensors in HeLa cells treated with 1 µM ionomycin on substrates with 1 and 40 kPa stiffness. Each line represents the mean value of normalized emission intensity for a group of 10 cells (n = 10), and the error bars reflect the standard error of the mean (SEM), illustrating the dynamic calcium changes in response to ECM stiffness and ionomycin treatment.

    Article Snippet: To determine the stiffness differences between the 1 and 40 kPa substrates, we initially employed Cyto-FAK (Addgene #78300) to observe focal adhesion kinase (FAK) activity across the two stiffness conditions (Supplementary Figure S1), as described by Seong et al. ( ).

    Techniques: Imaging